Taxonomic identification report

Sample ID: barcode84_contig1_PE250203-B_REP1_KAPA

Department of Agriculture, Fisheries and Forestry logo

[analysis description + link to github repo]

Facility Hogwarts
Analyst John Doe
Analysis started 2025-05-03 01:27:58
Analysis completed 2025-05-03 01:27:58
Wall time 0:0:0 hours

Result overview

Inconclusive

Outcome: The analyst should attempt subjective species identification at the genus level.

Reasoning: [Flag 1B] 2-3 candidate species matched with high stringency (identity ≥ 98.5%).

Preliminary morphology ID confirmed? NA

Inconclusive taxonomic identity (Flag 1B) Lycaenidae.

Taxa of interest detected? False

Flag 2B: Taxon of interest NOT detected in candidate species

Flag 5.1NA: Assessment of related species is only possible for taxa at rank genus/species

Flag 5.2NA: Assessment of related species is only possible for taxa at rank genus/species

Sample details

Locus COI
Preliminary ID Lycaenidae
Taxa of interest Chilades pandava
Country Australia
Host NA
Sample ID barcode84_contig1_PE250203-B_REP1_KAPA
Query DNA sequence

Analyst evaluation

Identification of candidate species

Flag 1B: The analyst should attempt subjective species identification at the genus level
2-3 candidate species matched with high stringency (identity ≥ 98.5%)

Candidate hits must meet ONE of these criteria:

Minimum alignment length 400bp
Minimum query coverage 85.0%

Candidate hits have then been classified as follows:

Classification Alignment identity Number of hits Number of species
STRONG MATCH ≥ 98.5% 34 2
MODERATE MATCH ≥ 93.5% NA NA
NO MATCH < 93.5%

Candidate species

Species Hits Identity E-value Database coverage
Euchrysops cnejus 33 100.0% 0.0
Lycaenidae sp. SSG-2014a 1 99.8% 0.0

Analyst evaluation

Taxa of interest (TOI)

This sections shows the taxa of interest (TOI) specified by the sample submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.

Taxon of interest Match rank Match taxon Match species Match accession Match identity Database coverage
Chilades pandava Did not match any candidate

Reference sequence source diversity

This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.

Analyst evaluation

Independent sources

Flag 4A: Reference sequences are from diverse sources and therefore likely to be reliable
Reasoning: Matching sequence records for this species are from >5 independent sources (found 12 sources)

12 Independent Sources

The matching reference sequences for this species have been annotated by 12 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession Automated Authors Title Journal
GQ129020 False Vila,R.
Bell,C.D.
Macniven,R.
Goldman-Huertas,B.
Ree,R.H.
Marshall,C.R.
Balint,Z.
Johnson,K.
Benyamini,D.
Pierce,N.E.
Phylogeny and palaeoecology of Polyommatus blue butterflies show Beringia was a climate-regulated gateway to the New World Proc. Biol. Sci. 278 (1719), 2737-2744 (2011)
GQ129020 False Vila,R.
Bell,C.D.
Macniven,R.
Goldman-Huertas,B.M.
Ree,R.H.
Marshall,C.R.
Balint,Z.
Johnson,K.
Benyamini,D.
Pierce,N.E.
Direct Submission Submitted (07-MAY-2009) ICREA and Departament de Genetica i Biologia Molecular, Universitat Autonoma de Barcelona, Edifici C, Bellaterra, Barcelona 08193, Spain

Source 2

Hit accession Automated Authors Title Journal
KF391539 False Hebert,P.D.
Dewaard,J.R.
Zakharov,E.V.
Prosser,S.W.
Sones,J.E.
McKeown,J.T.
Mantle,B.
La Salle,J.
A DNA 'barcode blitz': rapid digitization and sequencing of a natural history collection PLoS ONE 8 (7), E68535 (2013)
KF391539 False Hebert,P.D.N.
Dewaard,J.R.
Zakharov,E.V.
Prosser,S.W.J.
Sones,J.E.
Mckeown,J.T.A.
Mantle,B.
Lasalle,J.
Direct Submission Submitted (15-JUL-2013) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Rd E, Guelph, ON N1G2W1, Canada
KF390930 False Hebert,P.D.
Dewaard,J.R.
Zakharov,E.V.
Prosser,S.W.
Sones,J.E.
McKeown,J.T.
Mantle,B.
La Salle,J.
A DNA 'barcode blitz': rapid digitization and sequencing of a natural history collection PLoS ONE 8 (7), E68535 (2013)
KF390930 False Hebert,P.D.N.
Dewaard,J.R.
Zakharov,E.V.
Prosser,S.W.J.
Sones,J.E.
Mckeown,J.T.A.
Mantle,B.
Lasalle,J.
Direct Submission Submitted (15-JUL-2013) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Rd E, Guelph, ON N1G2W1, Canada
Show Hide 2 more publications...

Source 3

Hit accession Automated Authors Title Journal
KR868761 False Haldhar,S.M.
Behere,G.T.
Samadia,D.K.
Choudhary,B.R.
Bhargava,R.
Sahal,H.
Morphomatric analysis and development of DNA barcode of Euchrysops cnejus (Lepidoptera: Lycaenidae) on Indian beans in Rajasthan, India Unpublished
KR868761 False Haldhar,S.M.
Behere,G.T.
Samadia,D.K.
Choudhary,B.R.
Bhargava,R.
Sahal,H.
Direct Submission Submitted (25-MAY-2015) Division of Crop Improvement, ICAR-Central Institute for Arid Horticulture, Beechwal, Bikaner, Rajasthan 334006, India

Source 4

Hit accession Automated Authors Title Journal
HQ570952 False Bassett,Y.
Eastwood,R.
Sam,L.
Lohman,D.J.
Novotny,V.
Treuer,T.
Miller,S.E.
Weiblen,G.D.
Pierce,N.E.
Bunyavejchewin,S.
Sakchoowong,W.
Kongnoo,P.
Osorio-Arenas,M.A.
Comparison of rainforest butterfly assemblages across three biogeographical regions using standardized protocols J Res Lepid 44, 17-28 (2011)
HQ570952 False iBOL Data Release Unpublished
HQ570952 False Direct Submission Submitted (02-NOV-2010) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Rd West, Guelph, Ontario N1G2W1, Canada
HQ570948 False Bassett,Y.
Eastwood,R.
Sam,L.
Lohman,D.J.
Novotny,V.
Treuer,T.
Miller,S.E.
Weiblen,G.D.
Pierce,N.E.
Bunyavejchewin,S.
Sakchoowong,W.
Kongnoo,P.
Osorio-Arenas,M.A.
Comparison of rainforest butterfly assemblages across three biogeographical regions using standardized protocols J Res Lepid 44, 17-28 (2011)
HQ570948 False iBOL Data Release Unpublished
HQ570948 False Direct Submission Submitted (02-NOV-2010) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Rd West, Guelph, Ontario N1G2W1, Canada
HQ570953 False Bassett,Y.
Eastwood,R.
Sam,L.
Lohman,D.J.
Novotny,V.
Treuer,T.
Miller,S.E.
Weiblen,G.D.
Pierce,N.E.
Bunyavejchewin,S.
Sakchoowong,W.
Kongnoo,P.
Osorio-Arenas,M.A.
Comparison of rainforest butterfly assemblages across three biogeographical regions using standardized protocols J Res Lepid 44, 17-28 (2011)
HQ570953 False iBOL Data Release Unpublished
HQ570953 False Direct Submission Submitted (02-NOV-2010) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Rd West, Guelph, Ontario N1G2W1, Canada
HQ570965 False Bassett,Y.
Eastwood,R.
Sam,L.
Lohman,D.J.
Novotny,V.
Treuer,T.
Miller,S.E.
Weiblen,G.D.
Pierce,N.E.
Bunyavejchewin,S.
Sakchoowong,W.
Kongnoo,P.
Osorio-Arenas,M.A.
Comparison of rainforest butterfly assemblages across three biogeographical regions using standardized protocols J Res Lepid 44, 17-28 (2011)
HQ570965 False iBOL Data Release Unpublished
HQ570965 False Direct Submission Submitted (02-NOV-2010) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Rd West, Guelph, Ontario N1G2W1, Canada
Show Hide 10 more publications...

Source 5

Hit accession Automated Authors Title Journal
ON437086 False Lohman,D. Evolution and diversification dynamics of butterflies Unpublished
ON437086 False Lohman,D. Direct Submission Submitted (04-MAY-2022) Biology Department, City College of New York, 160 Convent Ave., Marshak J526, New York, NY 10031, USA

Source 6

Hit accession Automated Authors Title Journal
OL589388 False Vu,D.D.
Le,D.X.
Pham,P.M.
Dinh,D.T.
Le,S.X.
Pham,N.T.
Using DNA barcodes to identify animals on Truong Sa islands in Vietnam Unpublished
OL589388 False Vu,D.D.
Le,D.X.
Pham,P.M.
Dinh,D.T.
Le,S.X.
Pham,N.T.
Direct Submission Submitted (21-NOV-2021) Molecular Biology, Vietnam-Russia Tropical Centre, 63 Nguyen Van Huyen, Nghia Do, Hanoi 10000, Viet Nam

Source 7

Hit accession Automated Authors Title Journal
KJ402254 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402254 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402211 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402211 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402191 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402191 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402250 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402250 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402169 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402169 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402195 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402195 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402141 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402141 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402173 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402173 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402132 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402132 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402199 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402199 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402227 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402227 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402208 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402208 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402108 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402108 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402130 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402130 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402163 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402163 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402178 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402178 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402209 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402209 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402256 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402256 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
Show Hide 34 more publications...

Source 8

Hit accession Automated Authors Title Journal
KJ508008 False Stradomsky,B.
Fomina,E.
Comparative molecular genetic analysis of blue butterflies (Lepidoptera: Lycaenidae) Unpublished
KJ508008 False Stradomsky,B.
Fomina,E.
Direct Submission Submitted (27-FEB-2014) Laboratory of Molecular Biology, Institute of Arid Zones, Chekhov Str., 41, Rostov-on-Don, Rostov 344006, Russia

Source 9

Hit accession Automated Authors Title Journal
PP938841 False Debasree,B.
Rajasri,M.
Devaki,K.
Mohan Reddy,D.
Molecular Characterization of Euchrysops cnejus in Blackgram from Tirupati, Andhra Pradesh, India Unpublished
PP938841 False Debasree,B.
Rajasri,M.
Devaki,K.
Mohan Reddy,D.
Direct Submission Submitted (22-JUN-2024) Department of Entomology, Acharya NG Ranga Agricultural University, S. V. Agricultural college, Tirupati, Dept.of Entomology, SV Agricultural college, Tirupati, Tirupati, Andhra Pradesh, 517502-India, Tirupati, Andhra Pradesh 517502, India

Source 10

Hit accession Automated Authors Title Journal
OR140881 False Iqbal,W.
Malik,M.F.
Ghanavi,H.R.
Wahlberg,N.
Diversity of butterflies in Potohar Plateau, Pakistan Unpublished
OR140881 False Iqbal,W.
Malik,M.F.
Ghanavi,H.R.
Wahlberg,N.
Direct Submission Submitted (16-JUN-2023) Department of Zoology, University of Gujrat, Hafiz Hayat Campus, Gujrat, Punjab 50700, Pakistan

Source 11

Hit accession Automated Authors Title Journal
OQ152577 False Devi,A.
Walia,G.K.
Sidhu,A.K.
Direct Submission Submitted (02-JAN-2023) Zoology and Environmental Sciences, Punjabi University Patiala, Patiala, Patiala, PUNJAB 147002, India

Source 12

Hit accession Automated Authors Title Journal
MN685712 False Laskar,B.A.
Adimalla,H.
Prabhakaran,S.
Deepa,J.
Chandra,K.
Direct Submission Submitted (08-NOV-2019) Freshwater Biology Regional Centre, Zoological Survey of India, Plot 366/1 Hyderguda, Attapur, Hyderabad, Telangana 500048, India

Independent sources

Flag 4B: Reference sequence sources lack diversity and may therefore be unreliable
Reasoning: Matching sequence records for this species have only 1-5 independent sources (found 1 sources)

1 Independent Source

The matching reference sequences for this species have been annotated by 1 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession Automated Authors Title Journal
KJ402179 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402179 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India

Intraspecies diversity

This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.

Analyst evaluation

Click + drag to pan
Scroll to zoom in/out
Vertical space
Horizontal space

The phylogetic tree was constructed with FastME using the Neighbor-Joining method. Multiple-sequence alignment of the candidate reference sequences was performed using MAFFT. The visualization is rendered with TidyTree.

Taxonomy Check

The following resources can be used to ensure that the given taxonomy is legitimate and current.

Taxa Database
General GBIF
General ITIS
Mealybugs & scale ScaleNet database
Thrips Thripswiki
Spider Mites Spider Mites Database
Psocodea (Barklice, Booklice, and Parasitic Lice) Psocodea Species File Online
Orthoptera Orthoptera Species File Online
Drosophilidae TaxoDros
Diptera Catalog of the Diptera of the Australasian and Oceanian Regions
Systema Dipterorum
Aphids Aphid Species File
Ants AntWeb
AntCat
Lepidoptera (butterflies and moths) The Global Lepidoptera Names Index
Gracillariidae (primitive moths) Global Taxonomic Database of Gracillariidae
Pyralidae (pyralid moths) Global Information System on Pyraloidea
Tortricidae (tortrix moths) Tortricidae Resources on the Net